SrasV1, Main, Exploration, bibRecord, 005131

SARS coronavirus induces apoptosis in Vero E6 cells.

Identifieur interne : 005131 ( Main/Exploration ); précédent : 005130; suivant : 005132

SARS coronavirus induces apoptosis in Vero E6 cells.

Auteurs : Huimin Yan [République populaire de Chine] ; Gengfu Xiao ; Jiamin Zhang ; Yuanyang Hu ; Fang Yuan ; David K. Cole ; Congyi Zheng ; George F. Gao

Source :

Journal of medical virology [ 0146-6615 ] ; 2004.

RBID : pubmed:15170624

Descripteurs français

KwdFr :
- Animaux, Apoptose, Cellules Vero, Chromatine (ultrastructure), Coloration et marquage, Effet cytopathogène viral, Fluorescence, Fragmentation de l'ADN, Immunochimie, Microscopie électronique, Mitochondries (anatomopathologie), Mitochondries (ultrastructure), Noyau de la cellule (anatomopathologie), Noyau de la cellule (ultrastructure), Virus du SRAS (croissance et développement), Virus du SRAS (pathogénicité).
MESH :
- anatomopathologie : Mitochondries, Noyau de la cellule.
- croissance et développement : Virus du SRAS.
- pathogénicité : Virus du SRAS.
- ultrastructure : Animaux, Apoptose, Cellules Vero, Chromatine, Coloration et marquage, Effet cytopathogène viral, Fluorescence, Fragmentation de l'ADN, Immunochimie, Microscopie électronique, Mitochondries, Noyau de la cellule.

English descriptors

KwdEn :
- Animals, Apoptosis, Cell Nucleus (pathology), Cell Nucleus (ultrastructure), Chlorocebus aethiops, Chromatin (ultrastructure), Cytopathogenic Effect, Viral, DNA Fragmentation, Fluorescence, Immunochemistry, Microscopy, Electron, Mitochondria (pathology), Mitochondria (ultrastructure), SARS Virus (growth & development), SARS Virus (pathogenicity), Staining and Labeling, Vero Cells.
MESH :
- chemical , ultrastructure : Chromatin.
- growth & development : SARS Virus.
- pathogenicity : SARS Virus.
- pathology : Cell Nucleus, Mitochondria.
- ultrastructure : Cell Nucleus, Mitochondria.
- Animals, Apoptosis, Chlorocebus aethiops, Cytopathogenic Effect, Viral, DNA Fragmentation, Fluorescence, Immunochemistry, Microscopy, Electron, Staining and Labeling, Vero Cells.

Abstract

Severe acute respiratory syndrome (SARS) is an emerging infectious disease. Its etiological agent has been convincingly identified as a new member of family Coronaviridae (SARS-CoV). It causes serious damage to the respiratory system yet the mechanism is not clear. Infection-induced apoptosis or necrosis is suspected but no direct evidence for this yet exists. To date, Vero E6 cells are the only cell line that could be used to replicate the virus with obvious CPE (cytopathic effect) in vitro. It is known for some viruses (including members of family Coronaviridae) that CPE can be caused either by virus-induced apoptosis (active death) or cell necrosis (passive death). In this study, we examined the apoptosis in the SARS-CoV infected Vero E6 cells. Indeed, the results do show that the CPE was induced by apoptosis rather than necrosis, shown by typical DNA fragmentation, through the existence of apoptotic bodies and swollen mitochondria. This observation has some implications for the SARS-CoV pathogenicity: SARS-CoV does induce apoptosis in cell cultures and might have the same effect in vivo, responsible for the severe damage of the respiratory system.

DOI: 10.1002/jmv.20094
PubMed: 15170624

Affiliations:

Le document en format XML

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<author><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name>
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<author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Apoptosis</term>
<term>Cell Nucleus (pathology)</term>
<term>Cell Nucleus (ultrastructure)</term>
<term>Chlorocebus aethiops</term>
<term>Chromatin (ultrastructure)</term>
<term>Cytopathogenic Effect, Viral</term>
<term>DNA Fragmentation</term>
<term>Fluorescence</term>
<term>Immunochemistry</term>
<term>Microscopy, Electron</term>
<term>Mitochondria (pathology)</term>
<term>Mitochondria (ultrastructure)</term>
<term>SARS Virus (growth & development)</term>
<term>SARS Virus (pathogenicity)</term>
<term>Staining and Labeling</term>
<term>Vero Cells</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Apoptose</term>
<term>Cellules Vero</term>
<term>Chromatine (ultrastructure)</term>
<term>Coloration et marquage</term>
<term>Effet cytopathogène viral</term>
<term>Fluorescence</term>
<term>Fragmentation de l'ADN</term>
<term>Immunochimie</term>
<term>Microscopie électronique</term>
<term>Mitochondries (anatomopathologie)</term>
<term>Mitochondries (ultrastructure)</term>
<term>Noyau de la cellule (anatomopathologie)</term>
<term>Noyau de la cellule (ultrastructure)</term>
<term>Virus du SRAS (croissance et développement)</term>
<term>Virus du SRAS (pathogénicité)</term>
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</keywords>
<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Mitochondries</term>
<term>Noyau de la cellule</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term>
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<term>Mitochondria</term>
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<term>Mitochondria</term>
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<term>Apoptosis</term>
<term>Chlorocebus aethiops</term>
<term>Cytopathogenic Effect, Viral</term>
<term>DNA Fragmentation</term>
<term>Fluorescence</term>
<term>Immunochemistry</term>
<term>Microscopy, Electron</term>
<term>Staining and Labeling</term>
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<term>Coloration et marquage</term>
<term>Effet cytopathogène viral</term>
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<term>Fragmentation de l'ADN</term>
<term>Immunochimie</term>
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an emerging infectious disease. Its etiological agent has been convincingly identified as a new member of family Coronaviridae (SARS-CoV). It causes serious damage to the respiratory system yet the mechanism is not clear. Infection-induced apoptosis or necrosis is suspected but no direct evidence for this yet exists. To date, Vero E6 cells are the only cell line that could be used to replicate the virus with obvious CPE (cytopathic effect) in vitro. It is known for some viruses (including members of family Coronaviridae) that CPE can be caused either by virus-induced apoptosis (active death) or cell necrosis (passive death). In this study, we examined the apoptosis in the SARS-CoV infected Vero E6 cells. Indeed, the results do show that the CPE was induced by apoptosis rather than necrosis, shown by typical DNA fragmentation, through the existence of apoptotic bodies and swollen mitochondria. This observation has some implications for the SARS-CoV pathogenicity: SARS-CoV does induce apoptosis in cell cultures and might have the same effect in vivo, responsible for the severe damage of the respiratory system.</div>
</front>
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<tree><noCountry><name sortKey="Cole, David K" sort="Cole, David K" uniqKey="Cole D" first="David K" last="Cole">David K. Cole</name>
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<name sortKey="Hu, Yuanyang" sort="Hu, Yuanyang" uniqKey="Hu Y" first="Yuanyang" last="Hu">Yuanyang Hu</name>
<name sortKey="Xiao, Gengfu" sort="Xiao, Gengfu" uniqKey="Xiao G" first="Gengfu" last="Xiao">Gengfu Xiao</name>
<name sortKey="Yuan, Fang" sort="Yuan, Fang" uniqKey="Yuan F" first="Fang" last="Yuan">Fang Yuan</name>
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<country name="République populaire de Chine"><region name="Hubei"><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name>
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Serveur d'exploration SRAS

SARS coronavirus induces apoptosis in Vero E6 cells.

SARS coronavirus induces apoptosis in Vero E6 cells.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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